A Cautionary Note on the Use of Split-YFP/BiFC in Plant Protein-Protein Interaction Studies
نویسندگان
چکیده
منابع مشابه
A Cautionary Note on the Use of Split-YFP/BiFC in Plant Protein-Protein Interaction Studies
Since its introduction in plants 10 years ago, the bimolecular fluorescence complementation (BiFC) method, or split-YFP (yellow fluorescent protein), has gained popularity within the plant biology field as a method to study protein-protein interactions. BiFC is based on the restoration of fluorescence after the two non-fluorescent halves of a fluorescent protein are brought together by a protei...
متن کامل: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
The protein-nanoparticle interaction (protein corona) and its importance on the therapeutic application of nanoparticles
Nanobiotechnology has provided promising novel diagnostic and therapeutic strategies which capable to create a broad spectrum of nano-based imaging agents and medicines for human administrations. Several studies have demonstrated that the surface of nanomaterials is immediately coated with suspended proteins after contact with plasma or other biological fluids to form protein corona-nanoparticl...
متن کاملUsing the Protein-protein Interaction Network to Identifying the Biomarkers in Evolution of the Oocyte
Background Oocyte maturity includes nuclear and cytoplasmic maturity, both of which are important for embryo fertilization. The development of oocyte is not limited to the period of follicular growth, and starts from the embryonic period and continues throughout life. In this study, for the purpose of evaluating the effect of the FSH hormone on the expression of genes, GEO access codes for this...
متن کاملA cautionary note on the use of Ornstein Uhlenbeck models in macroevolutionary studies
Phylogenetic comparative methods are increasingly used to give new insights into the dynamics of trait evolution in deep time. For continuous traits the core of these methods is a suite of models that attempt to capture evolutionary patterns by extending the Brownian constant variance model. However, the properties of these models are often poorly understood, which can lead to the misinterpreta...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: International Journal of Molecular Sciences
سال: 2014
ISSN: 1422-0067
DOI: 10.3390/ijms15069628